A Surface Plasmon Resonance Sensor for the Detection of Deoxynivalenol Using a Molecularly Imprinted Polymer
نویسندگان
چکیده
The aim of the present work was to investigate the feasibility of applying the molecular imprinting polymer technique to the detection of the mycotoxin deoxynivalenol (DON) using a surface plasmon resonance (SPR) transducer. A molecularly imprinted polypyrrole (MIPPy) film was prepared via electropolymerization of pyrrole onto a bare Au chip in the presence of a template DON molecule. Atomic force microscope SPR analysis showed that the MIPPy film was deposited homogeneously on the Au surface, with a thickness of 5 nm. The MIPPy-SPR sensor exhibited a linear response for the detection of DON in the range of 0.1-100 ng/mL (R2 = 0.988). The selectivity efficiency of the MIPPy film for DON and its acetylated analogs 3-ADON and 15-ADON was 100, 19, and 44%, respectively. The limit of detection for DON with the MIPPy-SPR for a standard solution was estimated at >1 ng/mL. These results suggest that the combination of SPR sensing with a MIPPy film as a synthetic receptor can be used to detect DON.
منابع مشابه
Fabrication of a Selective and Sensitive Electrochemical Sensor Modified with Magnetic Molecularly Imprinted Polymer for Amoxicillin
A modified electrochemical sensor for the determination of amoxicillin (AMX) was reported in this paper. The magnetic molecularly imprinted polymer (MMIP) were suspended in AMX solution and then collected on the surface of a magnetic carbon paste electrode (CPE) via a permanent magnet, situated within the carbon paste electrode and then the voltammetry signals were recorded. It was confirmed th...
متن کاملUltrathin molecularly imprinted polymer sensors employing enhanced transmission surface plasmon resonance spectroscopy.
An ultrathin novel nanosensor (31.5 +/- 4.1 nm thick in the absence of analytes), employing a molecularly imprinted polymer as a recognition element for cholesterol and gold nanoparticle enhanced transmission surface plasmon resonance spectroscopy for detection, was constructed.
متن کاملA New Potentiometric Sensor for Determination and Screening Phenylalanine in Blood Serum Based on Molecularly Imprinted Polymer
Methods routinely utilized for detection of phenylalanine in new-born blood consist of enzymatic assays, lacking sensitivity and HPLC assays which are expensive and time-consuming to conduct. We, here, report for the first time, the construction of a phenylalanine sensitive electrode, on the basis of a selective molecularly imprinted polymer, offering sensitivity, economy and ease of use for th...
متن کاملIn Situ Voltammetric Determination of Promethazine on Carbon Paste Electrode Modified with Nano-sized Molecularly Imprinted Polymer
A precise and simple in-situ voltammetric measurement of promethazine, based on the nano sized molecularly imprinted polymer (nano-MIP) was introduced. The nano-MIP was synthesized utilizing vinyl benzene and Divinylbenzene as the functional monomer and cross-linker respectively, and via the micro-emulsion polymerization method in silicon oil. The MIP particles were then embedded in a carbon pa...
متن کاملA New Potentiometric Sensor for Determination and Screening Phenylalanine in Blood Serum Based on Molecularly Imprinted Polymer
Methods routinely utilized for detection of phenylalanine in new-born blood consist of enzymatic assays, lacking sensitivity and HPLC assays which are expensive and time-consuming to conduct. We, here, report for the first time, the construction of a phenylalanine sensitive electrode, on the basis of a selective molecularly imprinted polymer, offering sensitivity, economy and ease of use for th...
متن کامل